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Croda International Plc lipid splash mix
Lipid Splash Mix, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 91/100, based on 450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipid+standard+mixture/SPLASH+LIPIDOMIX+Mass+Spec+Standard/bio_rxiv__64898__2026__06__02__729405-248-9-15
Average 91 stars, based on 450 article reviews
lipid splash mix - by Bioz Stars, 2026-10
91/100 stars

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Related Articles

Liquid Chromatography with Mass Spectroscopy:

Article Title: Integration of Global Lipidomics and Gonad Histological Analysis via Multivariate Chemometrics and Machine Learning: Identification of Potential Lipid Markers of Ovarian Development in the Blue Mussel (Mytilus edulis)
Article Snippet: .. Raw data from LC-MS lipid adducts for POS and NEG were experimentally evaluated using the lipid standard mixture (Supplementary Table S2) purchased from Avanti Polar Lipids (www. avantilipids.com) and Matreya (www.matreya.com). .. Lipid IDs were obtained by searching for exact masses in the lipid mass spectra libraries LIPID MAPS (www.lipidmaps.org— accessed on 10 October 2024), the Human Metabolome Data Base (HMDB) (www.hmdb.ca— accessed on 10 October 2024) and Metlin (www.metlin.scripps.edu—accessed on 13 March 2020), as well as from an in-house lipid database derived from lipidomics studies on bivalves and other marine invertebrates, which served as a tool for the identification of unusual marine lipids [54,57–65].

Clinical Proteomics:

Article Title: Lipidomic Signature of Patients with Familial Hypercholesterolemia Carrying Pathogenic Variants Unveils a Cue of Increased Cardiovascular Risk.
Article Snippet: Additionally, C17 Ceramide (d18:1/17:0) (0.25 μg/mL) (Avanti Research, Merck, Milan, Italy) was incorporated. .. For lipid quantification, plasma samples were spiked with a commercial lipid standard mixture (SPLASHTM LIPIDOMIXTM Mass Spec, Avanti Research, Merck, Milan, Italy) which included (final concentration in samples reported in parentheses) 15:0–18:1 (d7) PC (16.07 μg/mL):15:0–18:1 (d7) PE (0.57 μg/mL), 15:0–18:1 (d7) PG (2.91 μg/mL), 18:1 (d7) LysoPC (2.55 μg/mL), 18:1 (d7) LysoPE (0.53 μg/mL), 18:1 (d7) ChE (35.61 μg/mL), 15:0–18:1 (d7) DG (0.94 μg/mL), 15:0–18:1(d7)–15:0 TG (5.73 μg/mL), and 18:1 (d9) SM (3.09 μg/mL). .. Additionally, C17 Ceramide (d18:1/17:0) (0.25 μg/mL) (Avanti Research, Merck, Milan, Italy) was incorporated.

Article Title: Lipidomic Signature of Patients with Familial Hypercholesterolemia Carrying Pathogenic Variants Unveils a Cue of Increased Cardiovascular Risk
Article Snippet: .. For lipid quantification, plasma samples were spiked with a commercial lipid standard mixture (SPLASH LIPIDOMIX Mass Spec, Avanti Research, Merck, Milan, Italy) which included (final concentration in samples reported in parentheses) 15:0–18:1 (d7) PC (16.07 μg/mL):15:0–18:1 (d7) PE (0.57 μg/mL), 15:0–18:1 (d7) PG (2.91 μg/mL), 18:1 (d7) LysoPC (2.55 μg/mL), 18:1 (d7) LysoPE (0.53 μg/mL), 18:1 (d7) ChE (35.61 μg/mL), 15:0–18:1 (d7) DG (0.94 μg/mL), 15:0–18:1(d7)–15:0 TG (5.73 μg/mL), and 18:1 (d9) SM (3.09 μg/mL). .. Additionally, C17 Ceramide (d18:1/17:0) (0.25 μg/mL) (Avanti Research, Merck, Milan, Italy) was incorporated.

Mass Spectrometry:

Article Title: Lipidomic Signature of Patients with Familial Hypercholesterolemia Carrying Pathogenic Variants Unveils a Cue of Increased Cardiovascular Risk.
Article Snippet: Additionally, C17 Ceramide (d18:1/17:0) (0.25 μg/mL) (Avanti Research, Merck, Milan, Italy) was incorporated. .. For lipid quantification, plasma samples were spiked with a commercial lipid standard mixture (SPLASHTM LIPIDOMIXTM Mass Spec, Avanti Research, Merck, Milan, Italy) which included (final concentration in samples reported in parentheses) 15:0–18:1 (d7) PC (16.07 μg/mL):15:0–18:1 (d7) PE (0.57 μg/mL), 15:0–18:1 (d7) PG (2.91 μg/mL), 18:1 (d7) LysoPC (2.55 μg/mL), 18:1 (d7) LysoPE (0.53 μg/mL), 18:1 (d7) ChE (35.61 μg/mL), 15:0–18:1 (d7) DG (0.94 μg/mL), 15:0–18:1(d7)–15:0 TG (5.73 μg/mL), and 18:1 (d9) SM (3.09 μg/mL). .. Additionally, C17 Ceramide (d18:1/17:0) (0.25 μg/mL) (Avanti Research, Merck, Milan, Italy) was incorporated.

Article Title: Lipidomic Signature of Patients with Familial Hypercholesterolemia Carrying Pathogenic Variants Unveils a Cue of Increased Cardiovascular Risk
Article Snippet: .. For lipid quantification, plasma samples were spiked with a commercial lipid standard mixture (SPLASH LIPIDOMIX Mass Spec, Avanti Research, Merck, Milan, Italy) which included (final concentration in samples reported in parentheses) 15:0–18:1 (d7) PC (16.07 μg/mL):15:0–18:1 (d7) PE (0.57 μg/mL), 15:0–18:1 (d7) PG (2.91 μg/mL), 18:1 (d7) LysoPC (2.55 μg/mL), 18:1 (d7) LysoPE (0.53 μg/mL), 18:1 (d7) ChE (35.61 μg/mL), 15:0–18:1 (d7) DG (0.94 μg/mL), 15:0–18:1(d7)–15:0 TG (5.73 μg/mL), and 18:1 (d9) SM (3.09 μg/mL). .. Additionally, C17 Ceramide (d18:1/17:0) (0.25 μg/mL) (Avanti Research, Merck, Milan, Italy) was incorporated.

Concentration Assay:

Article Title: Lipidomic Signature of Patients with Familial Hypercholesterolemia Carrying Pathogenic Variants Unveils a Cue of Increased Cardiovascular Risk.
Article Snippet: Additionally, C17 Ceramide (d18:1/17:0) (0.25 μg/mL) (Avanti Research, Merck, Milan, Italy) was incorporated. .. For lipid quantification, plasma samples were spiked with a commercial lipid standard mixture (SPLASHTM LIPIDOMIXTM Mass Spec, Avanti Research, Merck, Milan, Italy) which included (final concentration in samples reported in parentheses) 15:0–18:1 (d7) PC (16.07 μg/mL):15:0–18:1 (d7) PE (0.57 μg/mL), 15:0–18:1 (d7) PG (2.91 μg/mL), 18:1 (d7) LysoPC (2.55 μg/mL), 18:1 (d7) LysoPE (0.53 μg/mL), 18:1 (d7) ChE (35.61 μg/mL), 15:0–18:1 (d7) DG (0.94 μg/mL), 15:0–18:1(d7)–15:0 TG (5.73 μg/mL), and 18:1 (d9) SM (3.09 μg/mL). .. Additionally, C17 Ceramide (d18:1/17:0) (0.25 μg/mL) (Avanti Research, Merck, Milan, Italy) was incorporated.

Article Title: PPARγ Deficiency in SZ95 Sebocytes Elicits Redox Stress and Impairs the Sequestosome/Autophagy-Mediated Clearance of Oxidized Lipids
Article Snippet: High-purity ammonium acetate, ammonium formate, ammonium hydroxide (25%, w:v), and formic acid were purchased from Merck (Darmstadt, Germany). .. For internal standards, a labelled lipid standard mixture (EquiSPLASH; Avanti Polar Lipids) was used at a final concentration of 0.5%. ..

Article Title: A deep, quantitative lipid atlas of extracellular vesicles across multiple cell lines
Article Snippet: High-purity ammonium acetate, ammonium formate, ammonium hydroxide (25 %, w:v), and formic acid were purchased from Merck (Germany). .. For internal standards, a labelled lipid standard mixture (EquiSPLASH; Avanti Polar Lipids, AL, USA) was used at a final concentration of 0.5 %. .. For large-scale semi-quantitative analysis of lipid species another labelled lipid standard mix containing 69 deuterated lipids from various classes (UltimateSPLASH ONE, Avanti Polar Lipids) was used for calibration.

Article Title: Lipidomic Signature of Patients with Familial Hypercholesterolemia Carrying Pathogenic Variants Unveils a Cue of Increased Cardiovascular Risk
Article Snippet: .. For lipid quantification, plasma samples were spiked with a commercial lipid standard mixture (SPLASH LIPIDOMIX Mass Spec, Avanti Research, Merck, Milan, Italy) which included (final concentration in samples reported in parentheses) 15:0–18:1 (d7) PC (16.07 μg/mL):15:0–18:1 (d7) PE (0.57 μg/mL), 15:0–18:1 (d7) PG (2.91 μg/mL), 18:1 (d7) LysoPC (2.55 μg/mL), 18:1 (d7) LysoPE (0.53 μg/mL), 18:1 (d7) ChE (35.61 μg/mL), 15:0–18:1 (d7) DG (0.94 μg/mL), 15:0–18:1(d7)–15:0 TG (5.73 μg/mL), and 18:1 (d9) SM (3.09 μg/mL). .. Additionally, C17 Ceramide (d18:1/17:0) (0.25 μg/mL) (Avanti Research, Merck, Milan, Italy) was incorporated.

Solvent:

Article Title: PICALM Alzheimer's risk allele causes aberrant lipid droplets in microglia.
Article Snippet: Lipid extraction from iMGs iMGs carrying the PICALM risk allele, non-risk allele or risk-CRISPRa were grown in six-well plates until day 25. iMGs were dissociated with Accutase (07920, StemCell), collected and washed with PBS three times by centrifugation at 300g for 5 min. Lipids were extracted from iMGs as described previously80,81. .. In brief, a deuterated lipid standard mixture (330820, LM6003, 860739, 330727, 860657, 860658, Avanti Research) was added to iMGs equally and organic solvent (chloroform:methanol, 1:2 (v/v)) was added to iMG pellets and subjected to tip sonication, followed by vortexing and centrifugation to extract neutral lipid. ..

Article Title: PICALM Alzheimer’s risk allele causes aberrant lipid droplets in microglia
Article Snippet: iMGs carrying the PICALM risk allele, non-risk allele or risk-CRISPRa were grown in six-well plates until day 25. iMGs were dissociated with Accutase (07920, StemCell), collected and washed with PBS three times by centrifugation at 300 g for 5 min. Lipids were extracted from iMGs as described previously , . .. In brief, a deuterated lipid standard mixture (330820, LM6003, 860739, 330727, 860657, 860658, Avanti Research) was added to iMGs equally and organic solvent (chloroform:methanol, 1:2 (v/v)) was added to iMG pellets and subjected to tip sonication, followed by vortexing and centrifugation to extract neutral lipid. ..

Sonication:

Article Title: PICALM Alzheimer's risk allele causes aberrant lipid droplets in microglia.
Article Snippet: Lipid extraction from iMGs iMGs carrying the PICALM risk allele, non-risk allele or risk-CRISPRa were grown in six-well plates until day 25. iMGs were dissociated with Accutase (07920, StemCell), collected and washed with PBS three times by centrifugation at 300g for 5 min. Lipids were extracted from iMGs as described previously80,81. .. In brief, a deuterated lipid standard mixture (330820, LM6003, 860739, 330727, 860657, 860658, Avanti Research) was added to iMGs equally and organic solvent (chloroform:methanol, 1:2 (v/v)) was added to iMG pellets and subjected to tip sonication, followed by vortexing and centrifugation to extract neutral lipid. ..

Article Title: PICALM Alzheimer’s risk allele causes aberrant lipid droplets in microglia
Article Snippet: iMGs carrying the PICALM risk allele, non-risk allele or risk-CRISPRa were grown in six-well plates until day 25. iMGs were dissociated with Accutase (07920, StemCell), collected and washed with PBS three times by centrifugation at 300 g for 5 min. Lipids were extracted from iMGs as described previously , . .. In brief, a deuterated lipid standard mixture (330820, LM6003, 860739, 330727, 860657, 860658, Avanti Research) was added to iMGs equally and organic solvent (chloroform:methanol, 1:2 (v/v)) was added to iMG pellets and subjected to tip sonication, followed by vortexing and centrifugation to extract neutral lipid. ..

Centrifugation:

Article Title: PICALM Alzheimer's risk allele causes aberrant lipid droplets in microglia.
Article Snippet: Lipid extraction from iMGs iMGs carrying the PICALM risk allele, non-risk allele or risk-CRISPRa were grown in six-well plates until day 25. iMGs were dissociated with Accutase (07920, StemCell), collected and washed with PBS three times by centrifugation at 300g for 5 min. Lipids were extracted from iMGs as described previously80,81. .. In brief, a deuterated lipid standard mixture (330820, LM6003, 860739, 330727, 860657, 860658, Avanti Research) was added to iMGs equally and organic solvent (chloroform:methanol, 1:2 (v/v)) was added to iMG pellets and subjected to tip sonication, followed by vortexing and centrifugation to extract neutral lipid. ..

Article Title: PICALM Alzheimer’s risk allele causes aberrant lipid droplets in microglia
Article Snippet: iMGs carrying the PICALM risk allele, non-risk allele or risk-CRISPRa were grown in six-well plates until day 25. iMGs were dissociated with Accutase (07920, StemCell), collected and washed with PBS three times by centrifugation at 300 g for 5 min. Lipids were extracted from iMGs as described previously , . .. In brief, a deuterated lipid standard mixture (330820, LM6003, 860739, 330727, 860657, 860658, Avanti Research) was added to iMGs equally and organic solvent (chloroform:methanol, 1:2 (v/v)) was added to iMG pellets and subjected to tip sonication, followed by vortexing and centrifugation to extract neutral lipid. ..



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